Investigating the effects of temperature on

As the temperature increased from 0oC to 22oC on graph 1, and 0oC to 24oC on graph 2, the enzymes gain more kinetic energy, therefore colliding and binding with the substrate at a faster rate and also with more energy.

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Above the optimum temperature the rate decreases as more and more of the enzyme molecules denature. This is a significant difference, therefore the results for 35 degrees is largely inaccurate.

A cutting mat should be used as not to damage surfaces underneath by cutting or stained by any of the leaked pigment as a result of cutting the beetroot. Preparation a Make up lipase solution and suitable quantities of the other solutions.

Preparation a Make up lipase solution and suitable quantities of the other solutions. Beetroot, to use the cells to investigate how temperature alters the permeability of the cell membrane.

Diagram Method Firstly the equipment needed should be gathered in order to carry out the experiment. Only the weak hydrogen bonds are broken at these mild temperatures.

We could not fully control the light intensity, as even thought the room was blacked out to ensure no excess light contributed to the experiment, there were many other experiments occurring in the same room, therefore many other lamps. This will cause our measurements to be wrong when we are reading off the scale.

Dissolve 1 g in cm3 of IDA then make up to 1 litre with water. After around 25 degrees the line increases steadily till around 32 degrees on graph 2, and the same on graph 1, where after the steepness of the curve starts to decrease.

This is necessary as it can cause inaccurate results due to different number of leaves the different elodea contain. My prediction was close to my results, as I predicted the optimum temperature would be 40oC, although this is slightly lower that the results I obtained, it is still relatively near to 44oC and 43oC.

This is because on many occasions the bubbles did not show as one continuous bubble, but in fact many small ones. The above diagram shows a plan view of the placement of temperature and light treatments in the experiment and the diagram below shows the set up of each individual experimental unit.

I correctly predicted the rate of oxygen produced would rise, as temperatures get warmer, due to the increased kinetic energy of the enzyme rubisco.

The apparatus in the experiment is very important; gas leaks caused by old rubber tubing would affect the results we obtain massively, because the volume of C02 produced is what we are measuring. Other factors to test: This will affect the rate of photosynthesis, as each will have a different total surface area, therefore absorbing different amounts of light.

Analysis and conclusion The trend shown clearly depicts a correlation between percentage of light transmission and temperature applied to the specimen see graph and table below.

Should using towel in transferring hot water and ice. Sodium carbonate was added to speed up the rate of photosynthesis; the amount of this however was not measured. As the results show more beetroot pigment can leak out at higher temperatures, I can construe that cell membrane becomes more ruptured as temperature increases.

Take care with thermometers and brief students how to react if they are broken. Physiological and Morphological Responses of the Temperate Seagrass Zostera muelleri to Multiple Stressors: Investigating the Interactive Effects of Light and Temperature Paul H.

York, 1, 2, * Renee K. Gruber, 3, 4 Ross Hill, 5, 6 Peter J. Ralph, 1, 5 David J. Booth, 1 and Peter I. Macreadie 1, 5. To investigate the effects of temperature on the activity of lipase enzyme on milk which contain fats or lipids. Introduction: Enzyme is a kind of biological catalyst that made up of protein.

8. Place boiling tube in beaker of water on a tripod over a bunsen burner to heat to required temperature. 9. Heat beetroot at 20 oc, using a thermometer to measure the temperature and maintain for 2 minutes.

When heat reaches the required temperature, remove the boiling tube from the water bath using tongs because the tube will be hot. To investigate the effects of temperature I will be carrying out the following experiment on beetroot tissues to observe the movement of the red pigment out of the cells at different temperatures to see is the above theory is correct of temperature, and that at higher temperatures the red pigment will leak into the area surrounding the beetroot.

Investigating the Effects of Temperature on the Rate of Respiration of Blowfly Larvae This experiment was setup to investigate the effects of temperature on the rate of respiration of blowfly larvae.

Investigating the Effect of Temperature Essay

reactions, and are influenced by temperature just the same. In this lab, you will measure enzyme activity in a solution and measure the effect of five different temperatures: 30°C, 40°C, 50°C, 60°C, and 70°C.

Investigating the effects of temperature on
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Investigating effect of temperature on the activity of lipase | Nuffield Foundation